Elisa

Author: Prof. Dr. med. Peter Altmeyer

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Last updated on: 29.10.2020

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Synonym(s)

enzyme-linked immunosorbent assay; Enzymes linked immuno sorbent assay

Definition
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The term ELISA is an acronym for "enzyme-linked immunosorbent assay". The ELISA is an in vitro immunological test for the detection of biologically active substances in body fluids. The reaction between the antigen and the specific antibody is detected by the subsequent determination of an enzyme (e.g. horseradish peroxidase) bound to the antibody (photometric determination).

Note(s)
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With a comprehensive combination of 20 allergens and allergen mixes, the FastCheckPOC® 20 ATOPY(see http://www.dst-diagnostic.com/produkte/schnelltest-fastcheckpoc-20/panel/ below) provides a good basis for allergy screening. The multi-parametric immunoassay is reliable and easy to perform in practice. The following allergen mix is included in this immunoassay: Ragweed (Ambrosia artemisiifolia), Mugwort (Artemisia vulgaris), Timothy(Phleum pratense), Fragrant grass, Knobgrass, Ragweed (Anthoxanthum odoratum, Dactylis glomerata, Lolium perenne, Poa pratensis), rye (Secale cereal), birch ( Betula verrucosa), ficus/latex( Ficus benjamina, Hevea brasiliensis), cat scales (Felis domesticus), Dog scales ( Canis domesticus), house dust mites ( Dermatophagoides pteronyssinus, Dermatophagoides farina), egg white (Ovum gallinae), cow's milk ( Bos primigenius taurus), cod (Gadus morhua), Wheat (Triticum aestivum), peanut (Arachis hypogaea), soya (Glycine max), celery (Apium graveolens), almond (Prunus amygdalus ), walnut (Juglans regia), hazelnut (Corylus avellana).

Literature
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  1. Heinzerling LM et al (2009) GA(2)LEN skin test study I: GA(2)LEN harmonization of skin prick testing: novel sensitization patterns for inhalant allergens in Europe. Allergy 64:1498-1506

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Last updated on: 29.10.2020