ARISA

ARISA is the acronym for "automated ribosomal intergenic spacer analysis", a molecular technique for the rapid and cost-effective characterization of microbiota. The method is of great interest to both academic and industrial scientists.

See also T-RFLP and RFLP (restriction fragment length polymorphism)

ARISA as an automated ribosomal intergenic spacer analysis can be used to analyze the microbial ecology of e.g. biological products.

1. Fisher MM et al. (1999) Automated approach for ribosomal intergenic spacer analysis of microbial diversity and its application to freshwater bacterial communities. Appl Environ Microbiol 65:4630-4636.
2. Johnston-Monje D et al. (2020) Botanical microbiomes on the cheap: Inexpensive molecular fingerprinting methods to study plant-associated communities of bacteria and fungi. Appl Plant Sci. 8:e11334.
3. Porcellato D et al. (2014) Application of ARISA to assess the influence of salt content and cation type on microbiological diversity of Cheddar cheese. Lett Appl Microbiol 59:207-216.
4. Saro C et al. (2018) Comparison of automated ribosomal intergenic spacer analysis (ARISA) and denaturing gradient gel electrophoresis (DGGE) techniques for analyzing the influence of diet on ruminal bacterial diversity. Arch Anim Nutr 72: 85-99.