T-RFLP

Last updated on: 27.08.2024

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DefinitionThis section has been translated automatically.

In this technique, the target genes are amplified in the polymerase chain reaction (PCR) with one (or both) fluorescently labeled primer(s). The amplification products are digested with a restriction enzyme and analyzed by an automated sequencer.

General informationThis section has been translated automatically.

The T-RFLP technique of amplified restriction fragment length polymorphism is now also used to analyze polymorphism in bacterial and mycological samples (Masiga DK et al. 2000; Verrier J et al. 2019). The T-RFLP test has proven to be highly sensitive and specific and enables the rapid detection and direct identification of dermatophytes in medical practice (Verrier J et al. 2019).

LiteratureThis section has been translated automatically.

  1. Amélia Camarinha-Silva et al. (2012) Validating T-RFLP as a sensitive and high-throughput approach to assess bacterial diversity patterns in human anterior nares. FEMS Microbiology Ecology 79: 98-108.
  2. Didehdar M et al. (2016) Characterization of clinically important dermatophytes in North of Iran using PCR-RFLP on ITS region. J Mycol Med 26:345-350.
  3. Masiga DK et al. (2000) Amplified restriction fragment length polymorphism in parasite genetics. Parasitol Today 16:350-353.
  4. Rudner R et al. (1994) Determinations of restriction fragment length polymorphism in bacteria using ribosomal RNA genes. In: Methods in Enzymology. Volume 235:184-196
  5. Verrier J et al. (2019) PCR-terminal restriction fragment length polymorphism for direct detection and identification of dermatophytes in veterinary mycology. Med Mycol 57:447-456.

Last updated on: 27.08.2024