T-RFLP

In this technique, the target genes are amplified in the polymerase chain reaction (PCR) with one (or both) fluorescently labeled primer(s). The amplification products are digested with a restriction enzyme and analyzed by an automated sequencer.

The T-RFLP technique of amplified restriction fragment length polymorphism is now also used to analyze polymorphism in bacterial and mycological samples (Masiga DK et al. 2000; Verrier J et al. 2019). The T-RFLP test has proven to be highly sensitive and specific and enables the rapid detection and direct identification of dermatophytes in medical practice (Verrier J et al. 2019).

1. Amélia Camarinha-Silva et al. (2012) Validating T-RFLP as a sensitive and high-throughput approach to assess bacterial diversity patterns in human anterior nares. FEMS Microbiology Ecology 79: 98-108.
2. Didehdar M et al. (2016) Characterization of clinically important dermatophytes in North of Iran using PCR-RFLP on ITS region. J Mycol Med 26:345-350.
3. Masiga DK et al. (2000) Amplified restriction fragment length polymorphism in parasite genetics. Parasitol Today 16:350-353.
4. Rudner R et al. (1994) Determinations of restriction fragment length polymorphism in bacteria using ribosomal RNA genes. In: Methods in Enzymology. Volume 235:184-196
5. Verrier J et al. (2019) PCR-terminal restriction fragment length polymorphism for direct detection and identification of dermatophytes in veterinary mycology. Med Mycol 57:447-456.