RNA in situ hybridization is a method for the direct and specific detection of RNA in tissue, cells, cell compartments and chromosomes (see also FISH). In contrast to previous applications, RNA in situ hybridization allows the detection of individual molecules on formalin-fixed and paraffin-embedded tissue without additional effort in the laboratory, as the tests run on standard laboratory machines and can be evaluated under a light microscope. In contrast to molecular methods such as RT-PCR or whole-genome analyses, RNA-ISH preserves the tissue morphology and thus offers the decisive advantage of localizing the target cells in the tissue.
RNA in situ hybridization
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