Mastcell activation test

The "indirect mast cell activation test" (MAT) is a method for diagnosing immediate-type (type I) allergies or pseudo-allergic reactions. It uses an in vitro method to represent the in vivo reaction to allergen exposure. However, the test is not suitable for the detection of delayed-type hypersensitivity reactions (type IV reactions).

Mast cells from any donor or a mast cell line can be used for this in vitro procedure. Indirect sensitization is then carried out by incubation with the serum of the patient to be tested. The IgE antibodies present in the serum bind to the IgE receptors of the mast cells. They can now be detected in the same way as in the basophil activation test.

1. Glässner A et al. (2024) In vitro diagnostics of drug allergies. J Dtsch Dermatol Ges 22 (11):1529-1540.
2. Strohmeier d et al. (2017) Evaluation of the basophil activation test in the diagnosis of food and drug intolerances. Allerg J Int 26: 72-73.