DefinitionThis section has been translated automatically.
An IST genotype refers to the genetic variation in the Internal Transcribed Spacer (IST) region of an organism's ribosomal DNA (rDNA). The IST region is a section of DNA that lies between the ribosomal RNA genes (e.g. 18S, 5.89S, 28S). This region is often used in genetics to identify and compare species and to determine phylogenetic relationships.
General informationThis section has been translated automatically.
The IST region is highly variable, which makes it particularly useful for distinguishing closely related species.
The IST region is surrounded by conservative genes, which facilitates amplification (e.g. by PCR techniques).
The IST region is often used to identify fungi, plants and some bacteria.
An IST genotype describes the specific sequence variation in the IST region of an organism. By sequencing this region, species can be identified by comparison with databases such as GenBank, or UNITE. By identifying the IST genotype, populations or strains can be distinguished.
By identifying the IST genotype, evolutionary relationships can be analyzed. Differences in the IST sequence provide information on phylogenetic development.
