ROAT is the acronym for: Repeated open application test. This is an epicutaneous test procedure to clarify an unclear or questionable test reaction in the standard epicutaneous test in order to better assess the question of clinical relevance. In ROAT, the allergen is applied daily for 1 week without occlusion in a marked area on the inside of the upper arm. The test reaction is then assessed analogous to the conditions of the standard epicutaneous test.