Methylene blue staining

Author:Prof. Dr. med. Peter Altmeyer

All authors of this article

Last updated on: 29.10.2020

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DefinitionThis section has been translated automatically.

Simple microbiological staining technique for the detection of bacteria (e.g. gonococci).

ImplementationThis section has been translated automatically.

  • After the smear has been taken, spread a thin layer of secretion on the microscope slide.
  • Air-dry and fix the preparation (pull 3 times through the flame of a Bunsen burner).
  • Staining: Apply Löffler's methylene blue solution (3 drops are sufficient) to the slide and leave for 1-2 minutes.
  • Rinse under running tap water.
  • Dry slides immediately between filter paper and allow to air dry.
  • Result: Gonococci, leucocytes, cell membranes, granulae appear blue stained.

Authors

Last updated on: 29.10.2020

Author: Prof. Dr. med. Peter Altmeyer