DefinitionThis section has been translated automatically.
Simple microbiological staining technique for the detection of bacteria (e.g. gonococci).
ImplementationThis section has been translated automatically.
- After the smear has been taken, spread a thin layer of secretion on the microscope slide.
- Air-dry and fix the preparation (pull 3 times through the flame of a Bunsen burner).
- Staining: Apply Löffler's methylene blue solution (3 drops are sufficient) to the slide and leave for 1-2 minutes.
- Rinse under running tap water.
- Dry slides immediately between filter paper and allow to air dry.
- Result: Gonococci, leucocytes, cell membranes, granulae appear blue stained.
Prof. Dr. med. Peter Altmeyer