Immunoblot/western blot

Author: Prof. Dr. med. Peter Altmeyer

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Last updated on: 29.10.2020

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Definition
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Electrophoretically separated proteins are transferred onto a carrier material. These carriers are then incubated with serum and the specific binding of antibodies is detected with a labelled secondary antibody. Application for the detection of specific antibodies (HIV serology) in case of known antigen or for the identification of antigens if antibodies are available.

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Last updated on: 29.10.2020

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