Dapi

Author: Prof. Dr. med. Peter Altmeyer

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Last updated on: 29.10.2020

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Synonym(s)

4,6-Diamidino-2-phenylindoles, dihydrochlorides; CAS number: 128718-90-3

Definition
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DAPI is the acronym for 4,6-diamidino-2-phenylindole, dihydrochloride, a fluorescent dye that binds intensively to DNA, and to a lesser extent to RNA. Since DAPI diffuses through an intact cell membrane of living cells, it can be used in fluorescence microspy to mark living cells next to fixed cells. Furthermore, DAPI can be used to visualize mycoplasmas and viral DNA.

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Last updated on: 29.10.2020

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